A new book is out from Cambridge University Press, Dental Cementum in Anthropology, edited by Stephan Naji, William Rendu, and Lionel Gourichon. Although human teeth are not my area of expertise, I ended up coauthoring the twelfth chapter of the book, “Tooth cementum annulations method for determining age at death using modern deciduous human teeth: challenges and lessons learned”. Of all of my publications, this one is the hardest to write about. In part that’s because our original project failed, for various reasons that we document in the chapter, and the final publication is mostly a catalog of things not to do. But more importantly, it’s because Vicki Wedel, the lead author and my spouse of nearly 25 years, passed away unexpectedly last May.
I haven’t written here about Vicki’s passing because I’ve never been sure what to say. Other than two memorial ceremonies last year and a handful of Facebook posts in the month or so after, I haven’t talked about it in public at all. I hoped that I’d know what to say by the time that the book chapter was published, but here we are, and words still feel like grotesquely inadequate tools with which to sketch the horrifying suddenness and totality of the loss. I thought that time would dull the edge of grief, but it doesn’t hurt any less 10 months after, it just hurts less often. I haven’t become numb to any of the obvious triggers, I’ve just gotten good at side-stepping them. All that means is that it’s a cruel surprise when, at unpredictable and frequent intervals, grief sidles up and slips a dagger between my ribs.
Vicki and I met in high school, when we were both 16. We dated for five years, and got married when we were 21. Professionally, she was always ahead of me: she earned her bachelor’s degree first, and her master’s, and her doctorate; presented at a conference before I did, and traveled internationally, and published a journal article, and a book; got a tenure-track job first, and mentored a graduate student first. Far from being resentful, I was emboldened by her successes in every one of those arenas, and grateful for her example and her encouragement. She passed on May 15, 2021, three weeks short of our 25th wedding anniversary, and five months before our 30th anniversary as a couple.
As a forensic anthropologist, Vicki was frequently asked how she wanted to die. Her standard answer was that she wanted to go quietly in her sleep, at home, in clean clothes; to be found almost immediately by family; and to be conveyed rapidly to a funeral home. The timing was nothing that any of us had imagined or hoped for, but in the actual event she got everything she had wanted, and that is no small comfort. She went out at the apex of her personal and professional development, with no decline and no suffering, which is something that most of us will not get.
Vicki and I daydreamed of coauthoring papers together, and we always figured we’d get around to it eventually, although we both expected that any joint publications would be on dinosaur bone histology (she was the hard-tissue histologist, I would have supplied the dinosaurs). In the actual event, she was working on a project to determine age at death of human adolescents by counting cementum bands in deciduous teeth (‘baby teeth’), and she hit a wall transmuting the results into a discussion. I volunteered to help with that, and pretty soon I’d gotten sucked into being genuinely interested in the problem that she was up against.

The development and loss of deciduous teeth restrict cementochronology to the interval in which the root apex is complete. (Wedel et al. 2022: fig. 12.1)
I’ve written here before about the method of counting dental cementum bands, which are laid down annually, to determine age and season at death (this post). Vicki wanted to know if that method, which she’d used successfully on permanent teeth, would work on deciduous teeth. That turns out to be a surprisingly tricky problem, for several reasons. One of the foremost reasons is sampling. Human deciduous teeth have three fates:
- Most deciduous teeth complete development normally, which means that the roots are resorbed and the teeth fall out. The resorption of the root destroys the cementum bands, so there’s nothing to study.
- Some deciduous teeth are retained in the jaw instead of being resorbed, and usually these retained teeth are pulled by dentists when it becomes clear that they are not going to fall out on their own. Practically by definition, these retained teeth do not represent the typical course of development — not great when you’re trying to validate a method on ‘normal’ samples.
- Tragically, some deciduous teeth stop developing because they belong to people who die as children or adolescents. For reasons of privacy and respect for grieving loved ones these teeth are rarely used in research, and they don’t represent a controlled sample anyway. The remains of children from archaeological sites have the additional problem that there’s often no good independent line of evidence for age at death, which makes them useless for a validation study.
As we put it in the chapter, “normal, healthy deciduous teeth are unlikely to be extracted, and extracted deciduous teeth are therefore unlikely to be normal”.
We did have some deciduous teeth, culled from a sample of more than 1000 teeth collected by dentists at Creighton University in Omaha, Nebraska, and sent to Vicki by her collaborator, Ken Hermsen, who coauthored the chapter with us. Unfortunately, the methods that had worked so well for Vicki on adult teeth broke down when applied to deciduous teeth, in multiple ways that left us scratching our heads and chasing phantoms. I won’t go through the whole litany of failures here — it’s too depressing, and I already coauthored a whole chapter about it. Suffice it to say that peer review worked in this case, when an anonymous reviewer caught and called attention to our errors. We were ready to shelve the chapter, but lead editor Stephan Naji encouraged us to not let all our effort go to waste. About all we could do in the remaining time was catalog the stuff we’d done wrong, so…that’s the paper. It’s very much an ‘eating our vegetables’ affair, but hopefully it will steer future researchers away from the reefs that our original study foundered on. I’m grateful to Stephan for the opportunity to publish — not least because it would be my last chance to collaborate with my partner — and for the lovely words about Vicki that he wrote in the dedication of the book.
It is supremely bittersweet that Vicki and I finally got to coauthor something, only for it to come out when she’s no longer around to see it. It also hit me with unexpected force that with the publication of this book, Vicki’s scientific legacy is almost complete (there is one more collaboration, with folks other than me, that will hopefully still get published). Like many things related to her passing, those thoughts don’t point anywhere. There’s no neat resolution, no bow to tie things up with. Sometimes things just stop, awkwardly and before their time, and there’s nothing to do but go on.
Reference
Wedel†, V., Hermsen, K., & Wedel, M. 2022. Tooth cementum annulations method for determining age at death using modern deciduous human teeth: challenges and lessons learned. pp. 215-225 in Naji, S., Rendu, W., and Gourichon, L. (eds.), Dental Cementum in Anthropology. Cambridge University Press, Cambridge, UK. doi:10.1017/9781108569507.014
My spouse, Vicki, the other Dr. Wedel, is a physical and forensic anthropologist. And she’s one of a very small number of scientists who have (a) learned something new about the human body, and (b) used it to help identify dead people. And since that process involves the sciences of hard-tissue histology and skeletochronology–not to mention lots of dead folks–I reckon it might be of interest here. Hence this post.
This started about a decade ago, when Vicki was working on her PhD under Alison Galloway at UC Santa Cruz. Vicki worked with Alison on a ton of forensic cases, including some you probably heard of–they analyzed the remains of Laci Peterson and her unborn baby, Connor, for Scott Peterson’s murder trial. I had the unusual privilege of assisting a couple of times, on other cases, once to take some pictures in the lab while Vicki fished the skeleton out of the bag of skin that was all that was left of the body, and once to crawl around on my hands and knees picking human finger bones out of a muddy slough near Santa Cruz. All in all, I’m happy that my usual victims have been dead a lot longer.

CT reconstruction of skull with bullet holes. Courtesy of the National Library of Medicine
Incidentally, the only show with forensic content that Vicki will watch voluntarily is Dexter. She cannot stand CSI, NCIS, or the other “behind the scenes” forensic investigation shows. We’ve tried watching them, but the inaccuracies drive her crazy (paleo people: imagine getting the Clockwork Orange therapy and being forced to watch Clash of the Dinosaurs). Real cases are solved by teams of specialists, not two omnicompetent protagonists; it takes weeks or months, not half an hour; and if the forensics people carry guns, it’s because they know waaaay too much about how some very bad, very organized people dispose of bodies (the short answer is, not thoroughly enough*).
* Once a guy who was threatening to testify against a certain criminal organization was shot in the head, his body burned, and his burnt remains scattered along the side of the road. Vicki and Alison picked the bone shards out of the roadside gravel, identified some of them as bits of skull, and found bevelling diagnostic of ballistics trauma on some of those. The way the bone had shattered showed that the gunshot had been inflicted perimortem–around the time of death–and before the body was burned. Bottom line, whatever plan you have to get rid of the body, it is probably not going to be enough to keep someone like Vicki from figuring out how you did it. That much, the TV shows do get right.

Skull being cleaned by dermestid beetle larvae. Image from Wikipedia.
Not only is hard to really, truly get rid of a human body, it’s also hard to tell exactly when a person died, especially if all you have is a body in the woods. Insects are good–there’s a whole field of forensic entomology, whose practitioners age cadavers based on what insects are present and what stages of their life cycles they’re in. But what if all that is left is a pile of bones in the woods (which happens more often that you might think, and sometimes for completely innocuous reasons)? I’m preaching to the choir here, but bones can survive for a long time, so general wear-and-tear doesn’t tell you much. Rapetosaurus looks like it died last year.
There’s another side to this, which is figuring out how old someone was at the time of death based on their skeleton. Tooth eruption is good, and fusion of the epiphyseal growth plates, but both of those processes are basically done by the time people are in their mid-20s (teeth) to mid-30s (epiphyseal fusion). After that, there are methods based on the morphology of the auricular surface of the ilium and the public symphyses, but these only narrow things down to intervals of 5 to 15 years, and that’s a lot of missing persons reports to sift through. And none of the regular skeletal methods work past the age of 55 or 60. After that, no matter how healthy you are, the primary skeletal changes are attritional (i.e., you’re wearing out), and that process varies so much among individuals and populations that there are basically no predictive guidelines.
All of this was on Vicki’s mind when she was a grad student, so she was alert to anything that might help forensic anthropologists narrow down the possibilities for identifying dead folks. She was teaching in an osteology course and one of her students, Josh Peabody, brought up dental cementum increment analysis (DCIA), which is used in zooarcheology to determine the age and season at death of animal remains found at archaeological sites. Josh wanted to know if the method worked on humans.

Faunal bone from an archaeological dig. Image borrowed from the University of Copenhagen.
At the time–2004–DCIA was being tested for age at death in some historical human populations from archaeological sites, but no-one had tried using it for season at death. So Vicki and Josh set out to see if it would work.
Our teeth, like those of other mammals, are held in their sockets by periodontal ligaments. The periodontal ligament of each tooth attaches via Sharpey’s fibers to the dental cementum on the tooth root(s). Cementum is laid down in annual bands, so you can count the number of bands on a tooth, add the normal age at which that tooth erupts, and get a pretty tight estimate of when the animal died. So much for age at death, which was already being done on humans in a limited way in the early 2000s, albeit in archaeological rather than forensic contexts.
But wait, there’s more. Actually two bands of cementum are laid down every year–a dark band in the winter (roughly October to March) and a light band in the summer (roughly April to September). ‘Dark’ and ‘light’ describe the appearance of the bands under polarized light microscopy. In the summer months, the collagen fibrils that make up the cementum are aligned parallel to the tooth root, so more light comes through. In the winter, the collagen is aligned perpendicular to the root, so less light is transmitted, and the winter bands appear darker by comparison. So not only does the number of pairs of light-and-dark bands tell you the number of years since the tooth erupted, the color of the outermost band tells you in which six-month period the individual died, and the thickness of the outermost band might help you narrow that down even further.
At least, that’s how it works in other mammals. Would it hold up in humans? After all, we’re pretty good at adjusting our environments to suit us, rather than vice versa. If the winter-summer banding pattern was present in humans, it would be a huge boon to forensic science. Even people in their 40s and beyond with no very reliable skeletal indicators of age could be aged to within a year or two, and their season at death narrowed down to a 2-3 month window.
To find out, Vicki and Josh had a dentist in Santa Cruz collect 112 teeth pulled from patients over the course of a year (with full IRB approval and informed consent from the dental patients). For their purposes, a tooth pulled from a live person is just as good as one from a cadaver or skeleton–extraction kills the tooth as surely as death of the body. Better, even, in that it was easier to quickly get lots of teeth with very precise extraction data.
Vicki and Josh cut a few teeth together and they found dark and light bands right away. They presented those preliminary results at the American Academy of Forensic Sciences meeting in 2005. After that, Josh got busy with his own research, but Vicki pressed on (while finishing a dissertation on different project, and being a first-time mom).
If this was a movie, this is the part where there would be a montage of inspirational music to get us quickly past a lot of hard, boring work. Each of the 112 teeth had to be embedded in plastic, a section through the root cut out with a saw, that section mounted on a slide and ground down until it was translucent (this process will be familiar to bone histologists of all stripes, paleo or neo). Then Vicki had to go all the way around the perimeter of the each root to find the place where the cementum bands showed the most clearly, and count them. This part is trickier than it sounds, unless you’ve done some histo and know just how butt-ugly some sections can be under the scope.
The results? In the words of the Bloodhound Gang, which Vicki quotes in her DCIA talks, “You and me baby ain’t nothin’ but mammals”. Here’s the payoff graph:
The one out-of-place measurement was probably caused by the dark band not being thick enough to register clearly on the image.
Now that she knew that DCIA could be used to determine season at death in humans, Vicki started applying it in her forensic cases, of which there have been many. The vast majority of the work of forensic anthropologists is invisible to the public: after analyzing a set of remains, a forensic anthropologist writes a case report for whatever law enforcement office (or, much less frequently, law firm or other entity) brought them in, and that’s that. The case reports are almost always confidential, but they have to be written to exacting standards since they may be used as evidence in court. So forensic anthropologists spend a lot of time toiling over papers that hardly anyone gets to read.
However, sometimes a case is written up for journal publication–if it’s sufficiently novel or unusual, and if permission can be secured from all of the relevant parties. In 2008, Vicki was approached by the Merced County sheriff’s office to help try to identify the remains of a young woman who had been murdered in 1971. That’s the 37-year-old cold case mentioned in the title of this post, and rather than tell you about it, I’ll point you to Vicki’s case report (Wedel et al. 2013), published last month in the Journal of Forensic Identification and freely available here.

Vicki with the exhumed skeleton of Jane Doe. Photo by Debbie Noda of the Modesto Bee.
I wasn’t sure whether to post about this or not–as cool as they are, murder cases are not our normal stock in trade on this blog. What decided me was talking with Andy Farke. He read Vicki’s paper as soon as it came out, and he said that he really enjoyed getting to see how forensic anthropologists work in the real world. I sometimes take for granted that, since I am married to a forensic anthropologist, I get to see how this works all the time. But that’s a pretty rare experience–if paleontology is a small field, forensic anthropology is positively tiny. So if you want to see an example of the real science that CSI and the like are based on, here’s your window.
What’s next? Vicki has several validation studies on DCIA in progress, for which she and her collaborators have collected a much larger sample size–over 1000 teeth–to try to answer questions like: what tooth is best to use for DCIA? Should the histological sections be made longitudinally or transversely through the tooth root? Does cementum banding vary with latitude? And since banding patterns are reversed in the Southern Hemisphere, following the flip-flopped season, what happens at the equator? Watch this space, and keep an eye out for Vicki’s future publications–including a book due out next year–at her website, Bodies, Bugs, and Bones.
References
- Wedel, V.L. 2007b. Determination of season at death using dental cementum increment analysis. Journal of Forensic Sciences 52(6): 1334-1337.
- Wedel, V.L., G. Found, and G.L. Nusse. 2013. A 37 year-old cold case identification using novel and collaborative methods. Journal of Forensic Identification 63(1): 5-21.